Composite

Part:BBa_K1861040

Designed by: Vladyslav Vyshnevskyi   Group: iGEM15_FAU_Erlangen   (2015-09-18)

Fusion protein of SpyCatcher and RPD3 in an expression cassette

This part includes the rpd3 gene BBa_K1861401 fused C-terminally with the SpyCatcher BBa_K1159200 and cloned into our yeast expression cassette BBa_K1861010.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 826
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//chassis/eukaryote/yeast
//function/regulation/transcriptional
//regulation/negative
Parameters
None